The Development of a Manipulative Spectrophotometric Technique for the Determination of Aminexil in a New Hair Tonic Preparation

Abstract

A simple and economic ratio difference spectrophotometric method was developed and validated for the determination of Aminexil (AX), which is a new compound patented by L'Oreal, in the presence of niacinamide (NA) and pyridoxine (PD) in a newly introduced cosmetic preparation. The proposed method was found to be suitable for the determination of Aminexil in its laboratory prepared mixture in presence of the two other components; and in the commercial cosmetic preparation where the three components were co-formulated. The calibration curve was constructed with a concentration range from 2-10 μgmL-1 showing 100.22± 0.53 accuracy. The LOD and LOQ were found to be 0.53 and 1.6 μgmL-1 respectively. The method was validated according to ICH guidelines. The standard addition methods showed satisfactory mean recovery of 99.68±0.56.The obtained results were satisfactory enough for further statistical analysis, in comparison to the reported method. The proposed method had the advantage of being simple since no manipulation or complicated handling of the samples were needed, as well as economic, since there was no use of sophisticated instruments or expensive chemicals.